Skip to main content
Figure 2 | Genetic Vaccines and Therapy

Figure 2

From: The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events

Figure 2

The period of adsorption (a) and vector decay (b) were significant factors in determining transduction efficiency. The duration of the adsorption period was also shown to alter the transduction efficiency significantly. As the incubation period increased so did the number of transduced cells. At 4 h less than half of the active vectors had adsorbed to the cells. To estimate the t(1/2) of the vector system used here, we pre-incubated the inoculum for increasing periods of time before applying aliquots to the target cell monolayer. By applying the following equations VA = VAo exp (-kdt) and t(1/2) = ln(2)/kd to the data, {where VA is the concentration of active virus at time t, VAO is the initial concentration of active virus, and Kd is the virus decay rate constant}, the half-life of the vector was in the 8–9 hr range. The values represent mean ± SD (n = 4).

Back to article page

Genetic Vaccines and Therapy

ISSN: 1479-0556