Figure 4
From: HDAC inhibitor valproic acid upregulates CAR in vitro and in vivo
Effect of VPA on CAR transcriptional induction on tumors from patients with grade II cervical cancer treated with VPA. RNA samples before and after VPA treatment were obtained from a phase I cervical cancer trial. Pre-treatment biopsies were obtained the day before VPA treatment started. Patients were then started on oral magnesium valproate for a five-day period at 40 mg/kg. The post-treatment biopsy was taken at the sixth day post VPA treatment. RNA was extracted from the biopsy specimens for CAR RT-PCR semi-quantitative analysis. The GAPDH gene was used as loading control for semi-quantification analysis.