Genetic Vaccines and Therapy

Table 1 Effect of various pDNAX formulations on its persistence in beef cattle after i.m. administration

From: Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles

Beef cattle groups	Beef cattle ID code	Interval between 2^nd immunisation and slaughter (days)	pDNA copies at the injection site/500 μg DNA (n = 5)	pDNA copies opposite -to- injection site muscle (n = 4)	pDNA copies distant muscle (n = 3)	pDNA copies DLN^a total (n = 6)
pDNA	20087	242	< LQL (2); < DL (2); Neg. (1)	0/4	0/3	0/6
	20105	277	73.97; 35.59; < LQL (2); < DL (1)	0/4	0/3	0/6
	20080	284	< LQL (5)	0/4	0/3	0/6
DNA + B30-Nor-AbuMDP	20083	242	19.86; 16.23; 15.5; < LQL (2)	0/4	0/3	0/6
	28504	270	92.78; 29.25; 28;92; 24.04; 23.75	0/4	0/3	0/6
	20090	291	13.48; 12.73; 10.87; < LQL (1); Neg. (1)	< DL(1/4)	0/3	0/6
DNA:cationic liposome complex	20086	270	288; 220.08; 200.60; 30.07; < LQL (1)	0/4	0/3	0/6
	20089	277	228.9; 169.90, 134.70; 39.57; 39.00	0/4	0/3	0/6
	3654	291	149.3; 64.79; 46.60; 18.88; 18.73	0/4	0/3	0/6
	20081	298	< LQL (5)	0/4	0/3	0/6

The total amount 1000 μg of pDNAX in two equal doses was delivered into coccygeus muscle (injection site) as naked pDNAX, naked pDNAX + liposomal B30-norAbuMDP, and cationic liposome complex pDNAX:CDAN-DOPE. The level of pDNAX in the injection site, opposite-to-injection site, distant muscle tissues and draining lymph nodes was examined after 242–298 days after the second immunisation. Plasmid copies are expressed as mean plasmid copies per 500 ng of genomic DNA (MC/r) from duplicate QRTPCR assay. < LQL: below linear limit of quantification (10 copies/reaction), < DL: below detection limit (3 copies/reaction), Neg.: negative sample, ^aDLN- draining lymph nodes.

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