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Figure 1 | Genetic Vaccines and Therapy

Figure 1

From: Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice

Figure 1

Construction of recombinant plasmid OVA-Fc-pcDNA 3.1 (A) includes 1: DL 2 000 + DL 15 000 marker; 2: OVA-pMIgV/EcoR I + Xba I; 3:OVA-Fc-pcDNA/B gl II; 4: OVA-Fc-pcDNA/EcoR I; 5: PCR product of OVA-pcDNA 3.1 ; 6: λDNA/EcoR I + Hind III; 7: OVA-pMIgV/EcoR I +Bgl II; 8: OVA-Fc-pcDNA/EcoR I+Xba I; 9: OVA-pMIgV/Bgl II+Xba I. The expression of OVA-Fc fusion protein was detected in OVA-Fc-pcDNA 3.1-transfected CHO cells (B-1) and pcDNA3. 1-transfected CHO cells (B-2) by flow Cytometry. Immunization scheme of DNA vaccination prevention of avalbulin (OVA)-induced allergic airway inflammation (C).

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