Table 2 List of primers of each individual gene of HCV genotype 3a, Restriction sites worked successfully, Nucleotide position in full length sequence reference sequence of NZL1 was used and number of nucleotides in each amplified region
No. | Genes | Primer seq. 5'-3' | Restriction site | No. of Nucleotides (amplified region) |
|---|---|---|---|---|
1. | CORE-IS | ATGAGCACACTTCCTAAACCTCA | Hind III | Â |
2. | CORE-IAS | ACTGGCTGCTGGATGAATTAAGC | EcoR1 | 573 |
3. | E1-IS | CTAGAGTGGCGGAATACGTCTG | Hind III | Â |
4. | E1-IAS | GGCGACCCCTGAGAACATAACC | EcoR1 | 576 |
5. | NS2-IS | CTTTGGTCCCTAGCATTGC | Hind III | Â |
6. | NS2-IAS | CCTCACGGCCTAATCGTGC | EcoR1 | 642 |
7. | NS4A-IS | AGCACCTGGGTGTTGCTC | Hind III | Â |
8. | NS4A-IAS | GCACTCCTCCATCTCATCGT | EcoR1 | 168 |
9. | NS4B-IS | TCACAAGCTGCCCCATATATCG | Hind III | Â |
10. | NS4B-IAS | GCTACAAGGGCTTGGGTAGTC | Xba1 | 783 |