Figure 1
From: HCV entry receptors as potential targets for siRNA-based inhibition of HCV
Comparison of expression of CD81, LDLR, SR-BI and CLDN genes in HCV 3a and HCV 1a serum-infected Huh-7 cells. A) Gene expression of CD81, LDLR, SR-BI and CLDN1genes in Huh-7 infected with HCV serum of genotype 3a (S3a) and HCV serum of genotype 1a (S1a) as compared to normal serum (NSer). Cells were harvested and relative RNA determinations were carried out using semi-quantitative PCR. B) Relative gene expression of CD81, LDLR, SR-BI and CLDN1genes in Huh-7 infected with HCV serum of genotype 3a (S3a) and HCV serum of genotype 1a (S1a) as compared to normal serum (N) by real time PCR. All experiments were performed three times independently with triplicate samples in each. Error bars indicate the mean plus or minus SD. *p < 0.01 vs. normal