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Figure 5 | Genetic Vaccines and Therapy

Figure 5

From: Chitosan IFN-γ-pDNA Nanoparticle (CIN) Therapy for Allergic Asthma

Figure 5

CIN treatment induces apoptosis of goblet cells. BALB/c mice (n = 3) were sensitized and challenged with OVA as in Fig. 4 and then treated i.n. with CIN. Mice were sacrificed at 0, 3, 6, 12 and 24 h after CIN treatment and lungs were removed, sectioned and stained with hematoxylin/eosin (Fig. 5A), or unstained sections were analysed for apoptosis by TUNEL (terminal dUTP nick end labeling) assay (Fig. 5B). A final set of lung sections (Fig. 5C, 6 h time point) was stained for the goblet cell-specific protein Muc5a, and for apoptosis by the TUNEL assay. The first panel shows staining of nuclei with diamidinophenylindole (DAPI).

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