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Table 1 Transduction efficiency and gene expression analysis of MPSI-MSC and NIH3T3 cells with MLV-IDUA (I) and MLV-GFP (G) vectors

From: Treatment of adult MPSI mouse brains with IDUA-expressing mesenchymal stem cells decreases GAG deposition and improves exploratory behavior

  MPSI-MSCa NIH3T3b
  MOI 5.2 MOI 77 G418
  IDUA (U) c GFP (%)d IDUA (U)c GFP (%)d IDUA (U)c GFP (%)d
Control ND NE ND NE 23.8 ± 5.8 0
MLV-GFP NE NE 20.7 ± 21.6 NE 29.5 ± 29.5 100
1w (I/G) 2278 ± 249 8.9 ± 1.3 5340.4 ± 137.0 21.3 ± 6.6 NE NE
3-4w (I/G) 3016.5 ± 780 9.3 ± 2.2 59.7 ± 16.8 ND 5849.1 ± 851.6 100
6w (I/G) 15 ± 1.4 ND NE ND NE NE
  1. a MPSI-MSC were transduced with 5.2 or 77 MOI of MLV vectors and evaluated for transgene expression during 6 weeks; b NIH3T3 cells were transduced with MLV vectors and selected with G418 (these cells were used as a positive control cells); ND = non detectable; NE = non-evaluated; w = week; c IDUA activity is expressed in units of activity (nmols/h/mg of protein); d GFP-positive cells are expressed in percentage. All experiments were performed at least in duplicates. (I/G) means to monitor expression of IDUA or GFP, MSC were transduced with MLV-IDUA or MLV-GFP, respectively.