Treatment of adult MPSI mouse brains with IDUA-expressing mesenchymal stem cells decreases GAG deposition and improves exploratory behavior

Table 1 Transduction efficiency and gene expression analysis of MPSI-MSC and NIH3T3 cells with MLV-IDUA (I) and MLV-GFP (G) vectors

^a MPSI-MSC were transduced with 5.2 or 77 MOI of MLV vectors and evaluated for transgene expression during 6 weeks; ^b NIH3T3 cells were transduced with MLV vectors and selected with G418 (these cells were used as a positive control cells); ND = non detectable; NE = non-evaluated; w = week; ^c IDUA activity is expressed in units of activity (nmols/h/mg of protein); ^d GFP-positive cells are expressed in percentage. All experiments were performed at least in duplicates. (I/G) means to monitor expression of IDUA or GFP, MSC were transduced with MLV-IDUA or MLV-GFP, respectively.