Figure 1

Production of D6-AAV2 vectors and in vitro infection assay. (A) Electrophoresis and silver staining for the WT- and D6-AAV2 capsid proteins. The picture shows capsid proteins VP1, VP2, and VP3. The three capsid proteins of D6-AAV2 were present at similar relative quantities but had higher molecular weights than those of WT-AAV2. (B) Heparin agarose chromatography. When known amounts of WT- and D6-AAV2 were loaded onto a heparin agarose column, the D6-AAV2 viruses did not bind to the column and were present in the flowthrough fraction. (C) Infectivity in cells shown by the mCherry red fluorescence. HT1080 cells (a and e), HEK293 cells (b and f), C2C12 cells (c and g), and human chondrocytes (d and h) were infected with either WT- or D6-AAV2. Compared with the WT-AAV2-infected cells, the D6-AAV2-infected cells showed either weak fluorescence (HEK293) or no fluorescence (all other types of cells).