Skip to main content
Figure 3 | Genetic Vaccines and Therapy

Figure 3

From: A combination of intradermal jet-injection and electroporation overcomes in vivodose restriction of DNA vaccines

Figure 3

In vivo luciferase expression following Biojector plus EP injections with escalating doses of DNA. BALB/c mice were immunized once with Biojector followed by EP with 10-1000 μg of a luciferase-encoding plasmid (pVax-Luc) or empty vector diluted in 100 μl saline. A) 4, 8, 11, 18 and 25 days post DNA injections mice were injected ip with the D-luciferin substrate and expression of luciferase was monitored using the Xenogen In Vivo Imaging System (IVIS). Results are shown as mean values (n = 4) and error bars represent standard error of the mean. B) At day 25, spleens were collected and IFN-γ, IL-2 and IFN-γ/IL-2 responses were assessed by FluoroSpot. The H2-Kd restricted GFQSMYTFV (luciferase) and AMQMLKETI (Gag) peptides were used to assess luciferase-specific and unspecific cytokine secretion, respectively. Bars represent mean values for the GFQSMYTFV peptide. C) Correlation between in vivo expression at day 8 and frequency of GFQSMYTFV-specific IFN-γ, IL-2 and IFN-γ/IL-2 secreting splenocytes at day 25. *Significant difference (p < 0.05).

Back to article page