Table 1 Two paired ZFAs (pZFAs) required to engineer ZFNs that cleave within the human genomic context of the darc and glycophorin a genes
Zinc Finger Nuclease (ZFN) | Left Fn α-Helix; triplet | Right Fn α-Helix; triplet |
|---|---|---|
-darc | ||
ZFN-unknown-SP-5-1 | F1; SPSKLVR; (GCG) | F1; SKKSLTR; (GCC) |
1745 tGCCCTCTTCAGCATTGTGGTGCCc 1769 | F2; RQDNLGR; (GAG) | F2; EAHHLSR; (GGT) |
1745 aCGGGAGAAGTCGTAACACCACGGg 1769 | F3; QRNNLGR; (GAA) | F3; QPHGLAH; (TGT) |
-glycophorin- a | ||
ZFN-ASSEMBLY-SP-7-4 | F1; RSSHLKM; (AGG) | F1; RRVDLL; (GCC) |
8812 | F2; QRSDLTR; (GCT) | F2; RQDNLGR; (GGT) |
tCCTAGCTACTTGGGAGGCTGAGGCAg 8838 | ||
8812 aGGATCGATGAACCCTCCGACTCCGTc 8838 | F3; QSGTLTR; (GTA) | F3; VSNTLTR; (GCT) |