Primary data of GFP and eGFP transfected leukemic cell lines. Lymphocytes were gated based on their scatter profile (figures on the right) and cells were evaluated for transgene expression. a) GFP-expression of K562 cells. Cells were transfected with Ad-GFP at an MOI of 200 and assayed by flow cytometry 72 hours posttransfection. The overlay of the shaded histogram represents the background fluorescence of untreated cells. Positive transfected cells expressed intracellular green fluorescent protein. b) eGFP-expression of K562 cells 24 hours after nucleofection. c) eGFP-expression of HL60 cells 24 hours after nucleofection. Data are shown from one representative experiment.