Nucleofection mediated gene transfer in primary leukemic cells. eGFP expression in AML cells after exposure to optimized pulses. After 24 hours cells were harvested and analyzed by flow cytometric analysis. a) Representative flow cytomeric analysis for each of the three patients. Control cells were pulsed without DNA and showed no eGFP expression (left side); percentage of positive transfected cells is shown on the right. b) Gated and ungated transfection efficiencies of the primary AML cells. Percentage of dead cells was determined by PI staining. The figure represents data from two experiments, respectively. Data are presented as mean +/- standard error of the mean.