Figure 3
From: Novel non-viral method for transfection of primary leukemia cells and cell lines
Nucleofection mediated gene transfer in leukemia cell lines. eGFP expression in K562 and HL60 cells after exposure to the optimized pulses. After 24 hours cells were harvested and analyzed by flow cytometric analysis. GFP positivity was assayed in gated as well as in ungated cell populations. Control cells were pulsed without DNA and showed no eGFP expression. Percentage of dead cells was determined by PI staining. The figure represents data from five separate experiments, respectively. Data are presented as mean +/- standard error of the mean.