Figure 3

Western analysis of IL-15 expression in culture supernatants from SV(mIL-15)-transduced P815 cells P815 cells were transduced with SV(mIL-15) at a m.o.i. = 100. 1.5, 3 and 6 days post-transduction, culture supernatants were collected. Cultures were then stimulated with Con A and additional supernatants harvested 24 and 72 hours later. IL-15 secretion into supernatants was visualized by Western analysis. Recombinant human IL-15 (rhIL-15) was the positive control (non-adjacent lane). Supernatants from unstimulated cultures were tested in parallel (non-adjacent lane).