Skip to main content
Figure 1 | Genetic Vaccines and Therapy

Figure 1

From: Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer

Figure 1

Cytotoxic activity of immunological effector cells that had been co-cultured with DC against Dan-G pancreatic carcinoma cells. Immunological effector cells from a donor were co-cultured from days +10 to +14 with autologous DC cultures seven days of age, as described in materials and methods. DC were pulsed at day +7 with 200 ng/ml of tumor lysate. Cytotoxic activity at various effector to target cell ratios was measured by Europium release assay. Dan-G cells were used as targets. Data represent results of three separate experiments and are shown as mean. CIKs = CIKS cells only DC+CIKS = naive DC co-cultured with CIK cells DC+CIKSpIL-2 = naive DC co-cultured with CIK cells nucleofected with pIL-2 DC-Tu+CIKS = DC pulsed with tumor lysate and co-cultured with CIK cells DC-Tu+CIKSpIL-2 = DC pulsed with tumor lysate and co-cultured with CIK cells nucleofected with pIL-2 DC-Tu+CIKS (nucleofected) = DC pulsed with tumor lysate and co-cultured with CIK cells nucleofected without plasmid

Back to article page

Genetic Vaccines and Therapy

ISSN: 1479-0556