Figure 2
IL-2 gene expression analysis in human lymphoma cell lines by using an ELISA assay. (A) Daudi, Raji and LAM53 cells were infected with Ad.IL-2 or Ad.GFP at various MOI (0, 5, 100, 200). 72 h post-infection, IL-2 produced by Ad.IL-2-transduced Raji and Daudi cells at an MOI of 200 averaged 10.6 ng/ml/106 cells and 2.7 ng/ml/106 cells, respectively (mean ± SEM; n = 3). (B) Kinetic analysis of IL-2 production in Raji cells transduced at an MOI of 200 revealed peak secretions between day 2 and 3 and IL-2 was detectable until day 8 post-infection (mean ± SEM; n = 3). All experiments were performed in triplicates.