Figure 4

PBMC were incubated with cytokines (1–1000 pg/ml) either derived from supernatants of transduced Raji cells or recombinant with supernatants from Ad.GFP-transduced Raji cells as controls and assayed for their proliferative activity (mean ± SEM; n = 3). Adenoviral-expressed IL-2 (A) and IL-12 (B) led to dose-dependent increases in proliferation rates of PBMC. No significant difference between the effects of either cytokine was found. The proliferation effect could be blocked by addition of a neutralizing antibody against either cytokine. There was no significant difference between the effects of adenoviral-expressed or recombinant cytokines. MTT assays were performed in triplicates.