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Figure 5 | Genetic Vaccines and Therapy

Figure 5

From: Effects of recombinant adenovirus-mediated expression of IL-2 and IL-12 in human B lymphoma cells on co-cultured PBMC

Figure 5

Raji cells were transduced with Ad.IL-2 (MOI 200), Ad.Flexi-12 (MOI 200), or Ad.IL-2 and Ad.Flexi-12 (MOI 100 each) and co-cultured with PBMC for 72 h. Non-transduced (control) and Ad.GFP-transduced Raji cells were used as controls. Stimulated PBMC were harvested and assayed for their cytolytic activity against unmodified lymphoma cells by using an EuTDA non-radioactive cytotoxicity assay. Ad.IL-2-transduced lymphoma cells elicited a significant anti-tumor effect but not the combined effect of IL-2/IL-12 or IL-12 alone (mean ± SEM; n = 5; * p < 0.05).

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