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Table 2 Detection of transfected cells in bone marrow mononucleated cells at 5 day postinjection of eGFP conjugate

From: In vivogene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery

plasmid

eGFP

Cell population

Control serum

Unconjugate

Control conjugate

Conjugate

MNC

0

0

0

0

CD117-

0

0

0

0

CD117-/Sca1-

0

0

0

0

CD117+/Sca1-

0

0

0

4.7%

CD117+/Sca1+

0

0

0

2.8%

  1. The presence of transfected cells (eGFP positives) in bone marrow was investigated 5 days postinjection among mononucleated cells (MNC): CD117 negative cell population (CD117-), CD117/Sca1 negative cell population (CD117-/Sca1-), CD117 positive/Sca1 negative (CD117+/Sca1-) and CD117/Sca1 positive cell population (CD117+/Sca1+). In all cases no transfected cells were observed in the controls.
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Genetic Vaccines and Therapy

ISSN: 1479-0556