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Table 1 Different titres and MOI were obtained for the same vector stock when different numbers of target cells and volumes of inoculum were used. The number of positively transduced cells and thus the transduction efficiency, was also affected by the number of target cells in the transduction process, eg.: a thirty-fold increase in cell numbers resulted in a 53% decrease in efficiency. The transduction efficiency was highest with the smallest cell number and largest inoculum volume.

From: The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events

Titre TU/mL (followed by MOI)

Number of target cells

1 mL of VI Vol.

0.3 mL of VI Vol.

0.1 mL of VI Vol.

 

2.24 × 102 (0.224)

3.96 × 102 (0.119)

6.08 × 102 (0.061)

1 × 103

2.14 × 103 (0.071)

3.77 × 103 (0.038)

5.14 × 103 (0.017)

3 × 104

5.58 × 103 (0.056)

7.79 × 103 (0.023)

1.19 × 104 (0.012)

1 × 105

  1. TU – Transducing Unit; VI Vol – Volume of Inoculum.