Figure 7
From: Human cytomegalovirus plasmid-based amplicon vector system for gene therapy
Flow cytometry analysis of human cord blood CD34+ cells infected with CMV amplicon containing stocks, virus, or uninfected cell control. TN9-8GF5 amplicon (a,b), CMV-EGFP (RC2.7EGFP) virus (c), CMV (Towne) infected (d), or control uninfected human cord blood CD34 cells (e,f), were stained 36 hours post-infection with PE-antiCD34 antibody (a-e), or were left unstained (f), and were analyzed for two-color cytometry analysis using a FACS Calibur instrument. The dot-plots are generated using Cell Quest software and reveal the EGFP+ cells populations. Numbers in the upper right and lower right quadrants indicate percentage of the EGFP+CD34+ and EGFP+CD34- cells respectively. A frequency lower than 0.01% is considered negative.