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Figure 1 | Genetic Vaccines and Therapy

Figure 1

From: Immediate transfection of patient-derived leukemia: a novel source for generating cell-based vaccines

Figure 1

Kinetics of transgene expression in electroporated and cationic lipid transfected U2OS cells. (A) RFP expression over time in cultured U2OS cells when nucleofected with pDSRed2C-1 (RFP) plasmid. Black bars represent the percent cells expressing RFP corrected for the total number of cells transfected and gray bars represent the percent of viable cells expressing RFP. (B) RFP expression over time in cultured U2OS cells when novafected with pDSRed2C-1 (RFP) plasmid. Since there is no cell death associated with novafection, the gray and black bars both represent the percent cells expressing RFP from the total number of cells transfected. Autofluorescence (always <2%) detected from cells nucleofected or novafected with pcDNAHygro(-) control plasmid was subtracted from the experimental values. The black line in both A and B represents average cell number at each time point. All experiments were done in triplicate with the standard deviation indicated by the error bars.

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Genetic Vaccines and Therapy

ISSN: 1479-0556