Figure 5

Impact of cell cycle inhibition on gene vector expression. A) The average percentage of U2OS cells expressing vector-encoded CD137L 4 hours following nucleofection. The black bar represents cells nucleofected without plasmid, stippled bar represents untreated cells, and the gray bar represents mimosine treated cells. The error bars show the standard deviation from 3 separate experiments. B) Flow cytometric profile of propidium iodide stained mimosine treated U2OS cells (solid gray) and mimosine untreated U2OS cells (black line) prior to nucleofection of CD137L.