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Table 2 Transfection of Patient Leukemia. Patient leukemia cells were nucleofected with solution R, setting T20, as described in Methods. Patient diagnosis and specimen type are listed (PB, peripheral blood, BM, bone marrow). Relevant diagnostic criteria with respect to phenotype is also listed. Transfection efficiency is based upon the percentage of viable cells expressing RFP at 24 hours post-nucleofection.

From: Immediate transfection of patient-derived leukemia: a novel source for generating cell-based vaccines

Patient Sample

Diagnosis

Specimen

Phenotype

Transfection Efficiency

4

ALL

PB

45+/Tdt+/DR+/34+/10-/19+/20–24+/9+/56+

62.3

13

ALL

BM

45+/Tdt+/DR+/34-/10+/19+/20+/24+/9-/52+/22+

45.0

14

ALL

BM

45-/Tdt+/DR+/34–10+/19+/20dim/24+/9+

44.3

9

ALL-CD56

BM

45-/Tdt+/DR-/34+/10+/19+/20-/24-9+/56+

26.3

5

ALL-CD33

(a)PB

(b)BM

45+/Tdt+/DR+/34+/10+/19+/20–24+/9+/33+/117dim

23.0 (a)

13.8 (b)

11

ALL

BM

45+/Tdt+/DR+/34+/10+/19+/20-/24+/9+/13-/33-/117-/52+/25+

17.1

8

ALL

PB

45+/Tdt+/DR+/34-/10+/19+/20het/24+/9+/μ-/κ-/λ-

9.2

10

ALL-CD13/33

BM

45-/Tdt+/DR+/34+/10+/19+/20-/24+/9+/13+/33+/117-

8.0

3

ALL-CD15

BM

45-/Tdt+/DR-/34+/10-/19+/20-/24-9+15+/56+

5.9

7

ALL

BM

45-/Tdt+/DR+/34+/10+/19+/20–24+/9+

4.3

6

ALL

(a)PB

(b)BM

45-/Tdt+/DR+/34+/10+/19+/20-/24-/9+

3.4 (a)

1.0 (b)

12

T-ALL

BM

45+/Tdt+/DR+/34+/10-/19-/20-/24-/9-/13-/33-/117-/52+/7+/5+/2+/3-/4+/8-/1a-/TCRab-/ TCRgd-

2.9