Figure 5
From: A combined nucleocapsid vaccine induces vigorous SARS-CD8+ T-cell immune responses
SARS-CoV-NC specific CD8+ T cell responses in mice immunized with the candidate SARS vaccines. Fresh peripheral blood cells from immunized mice were stimulated with various antigens and stained for CD8, CD3 and IFN-γ with labeled monoclonal antibodies. After staining, flow cytometry was used to analyze the NC-specific CD8+ T cells. A negative control (without stimulation) and a positive control (phorbol myristate acetate + ionomycin) were included to control for the spontaneous production of IFN-γ. Cells were also stimulated with an irrelevant protein, HIV-1 gp120 (data not shown). A: Dot plots show results from individual representative animals from each group of mice. B: Results are shown as mean ± S.D. The symbol * indicates a significant difference (P = 0.01–000.1) compared to all other immunized groups. The symbol †indicates a significant difference (P = 0.026) compared to the control group.