Figure 1
From: A trial of somatic gene targeting in vivo with an adenovirus vector
Experimental steps to detect gene targeting in vivo. Gene targeting in vivo in liver cells was attempted after the delivery of donor DNA with an adenovirus vector. The gene with the required sequence change (lacZ-) on the lambda transgene in the mouse will be detected after its recovery in bacteriophage particles. Only lacZ-negative mutants can form plaques under the selective conditions.