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Table 2 Number of bacterial colonies obtained after transformation of tissue DNA after immunization with different doses.

From: Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery

pcDNA3-hsp65 doses

Time after immunization

Number of colonies/μg total DNAa

  

Muscle

DLN

BM

Liver

Spleen

Lung

Kidney

Thymus

100 μg

2

51

45

113.4

120.7

94.7

34.3

19.7

23.3

 

7

45.6

92.7

96.7

102.7

110.7

13.7

14

16.7

 

15

60.3

106.4

93

93.3

101.3

14

9.7

19.3

 

30

64.7

98.4

109

98.7

101

6.3

1.3

12.7

 

180

9.4

11.4

15.7

93.3

17.7

3

1

9.7

20 μg

2

2

3

1

0

3

0

0

0

 

30

0

0

0

0

0

0

0

0

 

180

0

0

0

0

0

0

0

0

  1. a This represents the total number of colonies obtained using 1 μg of total tissue DNA when the transformation efficiency was about 104 colonies/μg plasmid DNA (1 μg pcDNA3-Hsp65 wild type, data not shown). The total number of colonies is given as the median of values obtained in three independents transformation assay (three mice).