Figure 1

Dystrophin revertant fibres and transcripts in mdx mouse muscle. (A) A cluster of revertant fibres surrounded by a few single positive fibres in the mdx mouse. Tissue was immunostained for dystrophin using NCL-Dys 2. (B-D)Nested RT-PCR was carried out using Primer set 3 inner (Table 1) to study alternative splicing arrangements between exons 13–35. The bar (-) indicates the novel junctions in these transcripts. Unmatched boundary colours identify out-of-frame transcripts. The identity of all transcripts was confirmed by direct sequencing. (B)-Normal dystrophin, (C)-out-of-frame dystrophin transcripts and (D)-in-frame dystrophin transcripts. *Previously reported revertant transcripts *[26] and **[15].