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Figure 3 | Genetic Vaccines and Therapy

Figure 3

From: Induction of revertant fibres in the mdx mouse using antisense oligonucleotides

Figure 3

Induction of multiple exon skipping comparing two different AO cocktails. The cocktail used in (A) contains M23D(+02–18) and (B) contains M23D(+07–18), other AOs indicated in Table 2. Primer set 2 inner (Table 1) was used for nested PCR amplification where the intact product was 1357 bp long. Both (A) and (B) show the presence of generated multiple bands when the AO cocktail was used in vitro. Products were sequenced and transcripts identified. The major induced transcript of 217 bp, corresponds to the deletion of exons 19–25.

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