Figure 5

In vivo skipping of exons 19–25 induced with a PMO cocktail. As indicated by the 110 bp product, the PMO cocktail induced the removal of exons 19–25 at 2 and 4 weeks after a single 10 μg intramuscular injection in pups and adult mdx mice (A). Primer set 1 inner (Table 1) was used for nested PCR amplification. (B) Dystrophin expression 2, 4 and 8 weeks after intramuscular injection of PMO cocktail in both adults and pups. (C) Western blot analysis was performed one month after injection. A faint protein band of lower than normal dystrophin molecular weight is visible in lanes 6 and 7.