Figure 2
From: Characterization of the ribonuclease activity on the skin surface
Impact of a Cap structure at the 5'end of the substrate mRNA on the skin surface ribonuclease activity. Ribonuclease activity assay (negative images). Non-capped (-Cap) or capped (+Cap) mRNA coding for enhanced green fluorescent protein (eGFP, ~1 kb) was incubated for 15 min at 37°C with increasing concentrations (indicated by the wedge) of preparations from Homo sapiens hand surface or Mus musculus ear surface: final dilution 20× or 4×, respectively. Samples without ribonucleases are indicated by a dash.