Figure 2
From: HDAC inhibitor valproic acid upregulates CAR in vitro and in vivo
CAR mRNA transcriptional induction mediated by VPA. Given the potential use of VPA as a CAR upregulator in a clinical scenario, two potential VPA start-up times (12 or 24 hrs) prior to adenoviral gene therapy were evaluated. Twelve and twenty four hours post VPA pharmacological treatment, total mRNA was extracted, reverse transcription was performed and semi-quantitative PCR was done to assess changes on CAR mRNA levels as described in methods. The HeLa and MCF7 cancer cell lines treated with valproic acid displayed upregulation in CAR mRNA levels. The GAPDH gene was used as the loading control for semi-quantification analysis.