Figure 3

Effect of mice's age on priming by pVAXhsp65 (a) and on IL-4 production (b). BALB/c mice were primed with pVAXhsp65 at distinct ages (5, 12, 19 and 30 days) and boostered with this vaccine 4 weeks later. Antibody serum levels were evaluated by ELISA 2 weeks after the booster. Results represent the geometric mean ± SEM of 4 to 8 animals individually tested per group. Ability to produce IL-4 was tested in supernatants from splenic cells in mice with 5, 12, 19 and 30-day-old stimulated in vitro with ConA. Results represent the geometric mean ± SEM of 5 animals individually tested, except for the 5 days old group that was tested by a pool of cells. # p < 0.05 in comparison to 19 and 30 days; * p < 0.05 in comparison to the other groups.