Table 1 Median values of diameter distribution, encapsulation rate, and endotoxin levels in each formulation
Formulation | Composition | Average diameter (μm) | Encapsulation rate (μg/mg of microspheres) DNA – Protein | Endotoxin level (UE/mg) | |
|---|---|---|---|---|---|
Me-Control | - 100% of DNAv plus TDM-loaded PLGA 50:50 microspheres | 4.0 | 4.11 | - | 0.011 |
Me-DNAhsp65/TDM | - 100% of DNAhsp65 plus TDM-loaded PLGA 50:50 microspheres | 3.7 | 4.97 | - | 0.028 |
Me-Prime/boost | A mixture of PLGA microspheres containing: | ||||
- 50% of DNAhsp65 plus TDM-loaded PLGA 50:50 microspheres | 3.5 | 4.90 | - | 0.030 | |
- 50% rHsp65 protein plus TDM-loaded PLGA 75:25 microspheres | 2.4 | - | 1.70 | 0.053 | |