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Table 2 Bacterial replication in lungs from mice and guinea pigs vaccinated and challenged with M. tuberculosis

From: Comparison of different delivery systems of DNA vaccination for the induction of protection against tuberculosis in mice and guinea pigs

Vaccine formulationsa

Miceb

Guine-pigsb

No vaccination

6.12 ± 0.40

5.43 ± 0.38

BCG

3.25 ± 0.38*

3.80 ± 0.24*

DNAv (naked DNA)

6.02 ± 0.35

5.68 ± 0.29

DNAhsp65 (naked DNA)

4.76 ± 0.26*

4.90 ± 0.25*

Me-DNAhsp65/TDM

4.55 ± 0.28*

4.12 ± 0.25*

Me-Prime/boost

4.21 ± 0.30*

4.54 ± 0.27*

  1. aMice and guinea pigs (5–10 animals per group) were immunized by the following schedule: PBS (three intramuscular injection at 2-week intervals); DNA-hsp65 (three intramuscular injection of 100 ug DNA plasmid encoding M. leprae hsp65 gene at 2-week intervals); DNAv (plasmid DNA without the hsp65 gene administered at the same scheme for DNAhsp65); BCG (single intradermic injection of about 105 live bacteria in 50 ml saline); Me-DNAhsp65/TDM-loaded microspheres (single intramuscular injection of DNAhsp65 plus TDM-loaded PLGA 50:50 microspheres); Me-Prime/boost (single intramuscular injection of a mixture of PLGA microspheres as described in Table 1). Guinea pigs and mice were challenged by intratracheal route with 105 CFU of M. tuberculosis H37Rv, 30 days after the last immunization.
  2. bAnimals were killed 30 days after infection and the number of live bacteria in the lungs was determined as mean number of CFU ± SD (log10 values)/g lung tissue.
  3. * Indicate that the effects of vaccination were significant compared with data from animals not vaccinated and challenged with M. tuberculosis (Student's t-tests, P < 0.05).