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Figure 1 | Genetic Vaccines and Therapy

Figure 1

From: Immunological analysis of a Lactococcus lactis-based DNA vaccine expressing HIV gp120

Figure 1

The genetic anatomy of vaccine plasmids. Vaccine plasmids from L. lactis (pLL120) and E. coli (pEC120) with CpG motifs. The plasmids contain a similar expression cassette which carries the CMV promoter and the gp120 gene fused to the signal sequence tPA, and a transcription terminator (BGHpA). Intron A is included for efficient splicing. The pLL120 vector backbone contains the minimal theta type plasmid replicon encoding the repB protein and the hom-thrB genetic marker encoding two threonine biosynthetic enzymes homoserine dehydrogenase and homoserine kinase. The pEC120 vector backbone contains the colE1 origin of replication and the kanamycin resistance (KanR) genetic marker.

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