Figure 2
From: Immunological analysis of a Lactococcus lactis-based DNA vaccine expressing HIV gp120
Expression analysis of gp120. In vitro expression of gp120 protein from L. lactis (pLL120) and E. coli (pEC120) plasmids with and without CpG motifs. RIPA of culture supernatants of 35S-cys/met-pulsed HEK-293 cells transfected with the vaccine plasmids. Precipitation is done using pooled serum from HIV positive patients and the immunoprecipitates were analysed by SDS-PAGE. Positive control plasmid (Pos), untransfected cells (Neg), molecular marker (Marker). The gp120 is indicated.