Figure 2

Western blot analysis on eukaryotic cell-free in vitro expression of VP1 Protein using Transcend™ Chemiluminescent Translation Detection Systems (Promega, U.S.A.). Lane M shows a prestained protein molecular weight marker (Fermentas, Lithuania). Lane 1 to lane 4 represent the eukaryotic cell-free in vitro transcription/translation reactions of pVAX1/VP1-S, pVAX1VP1-4, pVAX1 and Luciferase T7 control DNA respectively. Lane 1 and lane 2 each shows a band of size 36 kD, indicating the translated VP1 protein. Lane 3 shows no band of translation product with using backbone vector pVAX1 alone. Lane 4 shows a band of size 61 kD, indicating the translated luciferase protein, which serve as a positive control for the eukaryotic cell-free in vitro expression reactions.