Figure 3From: DNA vaccine constructs against enterovirus 71 elicit immune response in miceAmplification of VP1 gene by RT-PCR. Lane M shows a 1 kb molecular weight marker (Fermentas, Lithuania). Lane 1 and lane 2 each shows a band of size 923 bp, indicating the amplified VP1 gene from extracted mRNA of Vero cells transfected with pVAX1/VP1-S and pVAX1/VP1-4 respectively. Lane 3 shows no RT-PCR product amplified from extracted mRNA of Vero cells transfected with backbone vector pVAX1 alone, which serve as a negative control. Lane 4 shows the PCR product of size 176 bp when backbone vector pVAX1 was amplified using T7 forward primer and BGH reverse primer. Lane 5 and lane 6 show no PCR product generated from the extracted mRNA of Vero cells transfected with pVAX1/VP1-S and pVAX1/VP1-4 respectively, when using T7 forward primer and BGH reverse primer.Back to article page