Figure 5
From: DNA vaccine constructs against enterovirus 71 elicit immune response in mice
Western blot analysis of transfected vero cells at 72 hours post transfection using rabbit anti-VP1 polyclonal antibody. Lane M shows a prestained protein molecular weight marker (Fermentas, Lithuania). Lane 1 and lane 2 each shows a band of size 36 kD, which represent the expressed VP1 protein in Vero cells transfected with pVAX1/VP1-S and pVAX1/VP1-4 respectively. Lane 3 and lane 4 represent Vero cells transfected with backbone vector pVAX1 alone and non-transfected Vero cells respectively, show no band of size 36 kD, indicating the absence of VP1 protein. Lane 5 represents the extracted protein of EV 71, shows a band of size 36 kD, which serve as a positive control.