Figure 8
From: DNA vaccine constructs against enterovirus 71 elicit immune response in mice
Virus neutralisation test of immunized mice seraon day 14. The neutralising activity of immunised mice sera against EV71 on day 14 was evaluated by virus neutralisation test using Vero cells. Figure 8a) and 8b) show almost 100% of CPE in Vero cells resulted by sera of mice immunized with pVAX1 and PBS (negative) respectively. Figure 8c) represents the virus control well with almost 100% CPE observed. Figure 8d) and 8e) showed the neutralizing activity of sera of mice immunized with pVAX1/VP1-S and pVAX1/VP1-4 respectively, where approximately 50% CPE was observed. Figure 8f) showed almost 100% neutralization of EV71-infected human positive serum where no CPE was observed. Figure 8g) represents the normal Vero cells that are not infected with EV71. All the dilution of serum samples in Figure 8a) to 8f) was at 1:32 and microscopy examinations were carried out at 100× magnification. Arrows indicate the CPE.