Figure 2
From: Improve protective efficacy of a TB DNA-HSP65 vaccine by BCG priming
Specific immune response detected in mice immunized or sham-immunized as described in Table 1. (A) IgG2a specific antibodies detected in serum collected before the first immunization (pre-immune) and 15 days after the last immunization. Detection of cytokines in supernatants of spleen cell cultures and IFN-gamma-producing cells (spleen cells) detected by ELISPOT. Groups of 7 mice were sacrificed 15 days after the last immunization, and the spleen cells were cultured in medium with or without rhsp65 (10 micrograms/well). (B) IFN-gamma, (C) IL-12, (D) IL-10, (E) IL-4 and (F) IFN-gamma spots. Bars represent the mean ± standard deviation. (A) ◆ all immunized-mice vs Infected. * BCGin/DNA vs BCGin, BCGsc, DNA-HSP65 and BCGsc/DNA. (B) ◆ all immunized-mice vs Infected. * BCGin/DNA vs BCGsc, BCGin, DNA-HSP65 and BCGsc/DNA. (C) ◆ BCGin, DNA-HSP65, BCGin/DNA and BCGsc/DNA vs Infected. * BCGin/DNA vs BCGsc, DNA-HSP65 and BCGsc/DNA. (D) ◆ BCGin, DNA-HSP65 and BCGin/DNA vs Infected. * BCGin/DNA vs BCGsc, DNA-HSP65 and BCGsc/DNA. # BCGin vs BCGsc. (E) ◆ BCGsc, DNA-HSP65, BCGin/DNA and BCGsc/DNA vs Infected. * BCGin/DNA vs BCGsc, BCGin and DNA-HSP65. (F) ◆ BCGin, DNA-HSP65, BCGin/DNA and BCGsc/DNA vs Infected.* BCGin/DNA vs BCGsc, BCGin, DNA-HSP65 and BCGsc/DNA. p < 0.05 was considered significant. Data are representative of two experiments.