Figure 3
From: Improve protective efficacy of a TB DNA-HSP65 vaccine by BCG priming
IFN-gamma, IL-12, IL-10 and TNF-alpha in lung homogenates from immunized, infected mice and infected mice after 30 days of the infection. (A) IFN-gamma; (B) IL-12; (C) IL-10 and (D) TNF-alpha. (E) Correlation between CFU numbers and IFN-gamma production (F) Correlation between IFN-gamma and IL-10 production. Groups of 7 mice were immunized according table I and 15 days after the last immunization, they were challenged with H37Rv. After 30 days of infection, the lungs were removed and the cytokine production in lungs homogenates was analyzed. Bars represent the mean ± standard deviation. (A) ◆ BCGsc, BCGin, DNA-HSP65, BCGin/DNA and BCGsc/DNA vs Infected mice. * BCGin/DNA vs BCGsc, BCGin, and DNA-HSP65. (B) ◆ All immunized-infected mice vs Infected mice. * BCGin/DNA vs BCGsc, BCGin, DNA-HSP65 and BCGsc/DNA. ● BCGin vs BCGsc. (C) ◆ All immunized-infected mice vs Infected mice. * BCGin/DNA vs BCGsc, BCGin, DNA-HSP65. (D) ◆ All immunized-infected mice vs Infected mice. p < 0.05 was considered significant. Data are representative of two experiments.