Skip to main content

Table 3 CD4+/CD8+ cell numbers and expression of CD44hiCD62Llo or CD44lo/CD62Lhi in the lungs of mice

From: Improve protective efficacy of a TB DNA-HSP65 vaccine by BCG priming

 

time after challenge

non-infected mice

infected mice

immunized with (below) and infected with H37Rv

    

BCGsc

BCGin

DNA-HSP65

BCGin/DNA

CD4 +

30 days

70 days

2.4 ± 0,6

1 ± 0,1

5 ± 0,8

1,6 ± 0,2

9 ± 0,7

1,7 ± 0,6

10,2 ± 0,5

2,18 ± 0,7

10,2 ± 0,8

1,64 ± 0,1

12,8 ± 1,5, *

2,8 ± 0,5, *

CD8 +

30 days

1,4 ± 0,2

2,5 ± 0,06

4,5 ± 0,4

5,9 ± 0,6

4,7 ± 1,5

6,2 ± 1,5

 

70 days

0,6 ± 0,3

1 ± 0,3

1 ± 0,4

1,3 ± 0,3

1 ± 0,1

1,4 ± 0,2

CD4 + /CD44 hi /CD62L lo

30 days

6,1 ± 2

12,8 ± 2,6

10 ± 4,7

10,7 ± 2,5

11,8 ± 2

15,3 ± 4

 

70 days

1,5 ± 0,5

3,6 ± 0,9

4,5 ± 1,4

6 ± 1

3,4 ± 0,9

9 ± 2,2, *

CD8 + /CD44 hi /CD62L lo

30 days

7,2 ± 1,4

14,6 ± 3,1

14,8 ± 6,4

12,8 ± 4

14,5 ± 1,3

20,8 ± 0,6*

 

70 days

1 ± 0,3

2,5 ± 0,7

2 ± 0,8

2,2 ± 1,7

2,7 ± 0,3

5,2 ± 0,5, *

CD4 + CD44 lo /CD62L hi

30 days

14 ± 1,5

17 ± 1,42

19,5 ± 2,6

36,8 ± 5,4,

24,6 ± 2,5

35,6 ± 4,3, *

 

70 days

12 ± 2,4

22,2 ± 1,27

26,2 ± 7,5

37,5 ± 5,

30 ± 3,8

42,4 ± 5,4, *

CD8 + CD44 lo /CD62L hi

30 days

15,3 ± 2

19,38 ± 1,7

21,9 ± 4,1

29 ± 2,8

18,42 ± 5,6

41,2 ± 6,5, *

 

70 days

8,7 ± 2

16 ± 4,4

24,3 ± 7,1

22,6 ± 1,1,

27,45 ± 6,1

35,2 ± 7,3, *

  1. Results were determined by flow cytometry. Number of CD4+ and CD8+ × 106 cells/mL. Expression of CD44hi/CD62Llo or CD44lo/CD62Lhi by CD4+ or CD8+ cells was calculated in percentage from dot plots analysis. Mice were immunized as described in table I and 60 days after the last immunization they were challenged with M. tuberculosis. 30 days: (CD4): All immunized-infected mice vs Infected mice. * BCGin/DNA vs BCGsc, BCGin and DNA-HSP65/(CD8): All immunized-infected mice vs Infected mice/(CD8+CD44hiCD62Llo): * BCGin/DNA vs BCGin/(CD4+CD44loCD62Lhi): All immunized-infected mice vs Infected mice. BCGin vs BCGsc. * BCGin/DNA vs BCGsc, DNA-HSP65/(CD8+CD44loCD62Lhi): BCGin and BCGin/DNA vs Infected mice. * BCGin/DNA vs BCGsc, BCGin and DNA-HSP65. 70 days: (CD4): BCGin/DNA vs Infected mice. * BCGin/DNA vs BCGsc and DNA-HSP65/(CD4+CD44hiCD62Llo): BCGin/DNA vs Infected mice. * BCGin/DNA vs BCGsc, BCGin and DNA-HSP65/(CD8+CD44hiCD62Llo): BCGin/DNA vs Infected mice. * BCGin/DNA vs BCGsc, BCGin and DNA-HSP65/(CD4+CD44loCD62Lhi): BCGin and BCGin/DNA vs Infected mice. BCGin vs BCGsc. * BCGin/DNA vs BCGsc and DNA-HSP65./(CD8+CD44loCD62Lhi): BCGin/DNA and BCGin vs Infected mice. BCGin vs BCGsc. * BCGin/DNA vs BCGsc, DNA-HSP65.