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Figure 1 | Genetic Vaccines and Therapy

Figure 1

From: Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivodendritic cells and T lymphocytes

Figure 1

Schematic representation of the FIV packaging constructs used. A) Parental clone pΔ00. B) Packaging construct pΔenv1; the 5' and 3' LTRs are replaced by CMVp and bovine growth hormone (BGH) poly A, respectively, ψ is partially deleted, and env is deleted by an internal 1 Kbp. C) Packaging construct pΔenv2; derived from pΔenv1 by removing the entire env except for the terminal ends overlapping the first exon rev and the Rev-responsive element (RRE) necessary for nuclear export of unspliced viral RNAs; D) Packaging construct pGP-CTE; obtained by deleting from within vif to BGH poly A, and replacing the Rev-RRE system with the Mason-Pfizer monkey virus constitutive transporting element (CTE); E) Packaging construct pGP-RRE; derived from pGP-CTE by replacing the CTE with the RRE retrieved by amplification from the parental clone pΔ00. For this construct, Rev was provided in trans.

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