Skip to main content
Figure 2 | Genetic Vaccines and Therapy

Figure 2

From: Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivodendritic cells and T lymphocytes

Figure 2

Schematic representation of the FIV vector constructs used. A) Parental clone pΔ00. B) Prototype LA34 vector: minimal, self-inactivating vector with both the U3 LTR domains deleted and devoid of all accessory and structural proteins except ψ, a 120 nt stretch at the of 5'-end gag containing the domains important for RNA encapsidation, and the RRE. MCS, the multiple cloning site, contains the CMVp-GFP cassette used as reported gene for all vectors. C) LA34-cPPT, vector derived from LA34 by inserting the central poly-purine tract (cPPT), important for nuclear import of the FIV preintegration complex, between RRE and MCS. D) LAW34, vector obtained by inserting the woodchuck post-trascription regulatory element (WPRE) in LA34 downstream MCS. Variant vectors having a longer (310 nt) gag fragment (LA34-L and LAW34-L, respectively) were also produced but are not shown. Total size indicates the number of nucleotides of the vector without the CMVp-GFP cassette.

Back to article page

Genetic Vaccines and Therapy

ISSN: 1479-0556