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Table 1 Vector titers generated from transfected 293T or CrFK cellsa

From: Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivodendritic cells and T lymphocytes

Vector/Env used for pseudotyping

Vector produced in

Vector titer (RNA copies/ml)

Concentration

Transduction units/mlb

LA34/VSV-G

CrFK

8.5 × 109

None

3.8 × 106

 

293T

1.8 × 109

None

1.2 × 106

LAW34/VSV-G

293T

5.0 × 109,c

None

2.5 × 107

  

2.1 × 1010,c

Ultracentrifugation

3.0 × 107

 

293T

7.0 × 109

PBd

3.0 × 107

LAW34/RD114/TR

293T

1.4 × 1010,c

Ultracentrifugation

6.0 × 107

  

4.0 × 109,c

PB

1.2 × 108

  1. a Titers measured in supernatants collected 2 days post-transfection, average of three independent experiments.
  2. b Measured in 293T cells.
  3. c Same supernatant, titrated as such or after 10 fold concentration by ultracentrifugation.
  4. d Polybrene.