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Table 2 LA34/VSV-G pseudotyped particles generated 2 days post-transfection in 293T cellsa

From: Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivodendritic cells and T lymphocytes

Packaging construct used for vector production

p25 optical density

Vector RNA copies/ml

Transduction units/mlb

pΔenv1

2.24

9.3 × 109

6.4 × 106

pΔenv2

>2.50

3.0 × 109

3.4 × 106

pGP-CTE

1.09

2 × 106

1.0 × 103

pGP-RRE c

1.92

8.1 × 108

3.0 × 105

  1. a Average of three independent experiments, mean efficiency of transfection 80% (range 75–90%);
  2. b Titer measured in the indicated cells 2 days post-transduction;
  3. c Rev provided in trans by cotransfection of pcDNA-Rev.
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Genetic Vaccines and Therapy

ISSN: 1479-0556