Figure 10
ΔRR vaccination is associated with IL-10 production by microglia and inhibition of TNF-α. Sprague Dawley rats were mock treated with PBS or treated with ΔRR or ΔPK [50 μl (2.5 × 10 6pfu)] by intranasal delivery as described in Materials and Methods. They were given KA by i.p. injection (24 hrs later) and the brains were collected 2 days later. Serial sections were stained with FITC-labeled ICP10 antibody, Texas Red-labeled IL-10 + FITC-labeled CD11b antibodies, or Texas Red-labeled TNF-α antibody. Blue staining is DAPI. The numbers of staining cells were counted in 3 randomly selected fields of 29 μm2 (at least 250 cells) from 5 serial sections for all animals and the data are expressed as % staining cells ± SEM relative to total DAPI stained cells.